Knowledge-enriched characterization of mobile populations. Morphological data from images can incorporate to the richness of flow cytometry details. One example is, the determine displays an normally typical apoptosis assay employing Annexin V and PI, introducing mobile imaging to characterize cells in Each and every inhabitants to reveal morphologically unique options. These insights couldn't are already acquired from multiplex staining by itself.

As samples are obtained over the Attune CytPix Flow Cytometer, the substantial-velocity brightfield digicam captures and stores pictures of detected activities at a amount up to six,000 illustrations or photos per 2nd, according to the flow charge and picture dimension. For better flexibility, Attune Cytometric Application lets you regulate the impression seize frequency as desired and select a certain gate for graphic capture, if essential.

Blend acoustic concentrating know-how with superior-parameter Assessment for versatile spectral unmixing and standard workflows.

The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic focusing by using a high-speed brightfield camera for simultaneous large throughput flow cytometry and higher resolution brightfield imaging.

"A picture isn't really rather worthy of one thousand dots, nonetheless it truly helps you to see photographs. It is excellent to see photographs that correlate with staining. As an example, AnnexinV-optimistic cells which are morphologically different than nutritious cells, or CD14+ monocytes that happen to be more substantial and more textured than CD3+/CD4+/CD14- lymphocytes.

Furthermore, it's illuminating to find out the number of doublets slip by way of standard singlet gates, and good to verify the standing of unforeseen double-constructive functions in my analysis."

Pair your Attune flow cytometer with an autosampler for increased workflow performance, and insert robotic integration for max throughput.

Our experts will style and design panels for you as often times you need—free of charge, no product invest in desired.

Early indications of this contamination had at first been dismissed as debris. Backgating to discover new populations. Backgating imaged cells around the Attune CytPix Flow Cytometer also permits you to use morphological attributes to find out exciting subpopulations that might not be evident from flow cytometry knowledge by yourself.

Buyers can check out occasion illustrations or photos in authentic-time to verify sample quality, improve protocols, and report the morphology of each mobile populace discovered for Investigation. Imaging capture also Advantages from acoustic concentrating, which helps to place the cells making sure that a pointy, centered picture is received.

Audio waves permit precise more info mobile alignment, causing Fantastic details good quality and ~10X faster sample flow charges.

Software Observe: Automatic image Assessment reduces user-to-person variability in flow cytometry gating tactics

Leverage the power of State-of-the-art engineering and innovation to accelerate your discoveries. Have faith in from the Attune Flow Cytometer loved ones to provide the dependability, speed, and accuracy that established your get the job done apart.

Flexible payment system from Agilent Economical Methods permit you to obtain the important instrumentation you will need for rapid project, or to improve your lab.

At the guts of each and every Attune Flow Cytometer lies our innovative acoustic focusing technology, which utilizes audio waves to meticulously align cells within the flow mobile. This impressive strategy surpasses standard hydrodynamic strategies, causing faster processing speeds, Fantastic information high-quality, and greater sample throughput.

The Attune Xenith cytometer is a large-parameter spectral analyzer, giving both equally spectral unmixing and traditional flow abilities for extensive and multipurpose mobile Examination.

The Attune CytPix Flow Cytometer enables users to visualize samples throughout acquisition, verify the accuracy of gating strategies, and assistance outcomes with reliable documentation for publication needs.

What seemed to be a homogeneous populace is definitely a lot more numerous—an Perception that ought to be considered when interpreting success.